Mycoplasma Detection Kit from SouthernBiotech

Product Specs
ItemMycoplasma Detection Kit
CompanySouthernBiotech
Price Pricing Info Supplier Page View Company Product Page
Catalog Number13100-01
ApplicationsPCR
Quantity50 Reactions

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SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) Structure and mechanism of the human CTDNEP1-NEP1R1 membrane protein phosphatase complex necessary to maintain ER membrane morphologybioRxivNovember 25, 2023Shujuan Gao, Jake W. Carrasquillo Carrasquillo Rodríguez, Shirin Bahmanyar, Michael V. AirolaCells were tested for mycoplasma upon initial thaw and generation of new cell lines (Southern Biotech 13100–01), and untreated cells were continuously profiled for contamination by assessment of extranuclear DAPI/Hoechst

(2) The Axin scaffold protects the kinase GSK3β from cross-pathway inhibitionElifeAugust 7, 2023Maire Gavagan, Noel Jameson, Jesse G ZalatanRRID:CVCL_0045). Cell cultures were tested monthly for mycoplasma contamination (Southern Biotech #13100–01). In vivo cell culture experiments The Axin open reading frame (Gavagan et al., 2020) was cloned into

(3) Small-Molecule–Mediated Stabilization of PP2A Modulates the Homologous Recombination Pathway and Potentiates DNA Damage-Induced Cell DeathMolecular Cancer TherapeuticsMay 4, 2023Rita A. Avelar, Amy J. Armstrong, Gracie Carvette, Riya Gupta, Noah Puleo, Jose A. Colina, Peronne Joseph, Alexander M. Sobeck, Caitlin M. O'Connor, Brynne Raines, et al.studies. All cell lines were tested for the presence of Mycoplasma using a PCR-based kit (Southern Biotech 13100–01) and all tested negative before proceeding with the experiment. These studies were approved by IACUC. CRISPR Cas9

(4) Hyperextended telomeres promote formation of C-circle DNA in telomerase positive human cellsJournal of Biological ChemistryMay 1, 2023Celina Y. Jones, Christopher L. Williams, Sara Priego Moreno, Danna K. Morris, Chiara Mondello, Jan Karlseder, Alison A. BertuchCytogenetics and Cell Authentication Core and were routinely checked for mycoplasma (SouthernBiotech #13100-01). Cen3tel cells were authenticated as having distinct STR profiles from all other cell lines and being

(5) Hyperextended telomeres promote C-circle formation in telomerase positive human cellsbioRxivJanuary 31, 2023Celina Y. Jones, Christopher L. Williams, Sara P. Moreno, Danna K. Morris, Chiara Mondello, Jan Karlseder, Alison A. BertuchCytogenetics and Cell Authentication Core and were routinely checked for mycoplasma (SouthernBiotech #13100–01). Cen3tel cells were authenticated as having distinct STR profiles from all other cell lines and being

(6) Doxorubicin-Loaded Polymeric Meshes Prevent Local Recurrence after Sarcoma Resection While Avoiding CardiotoxicityCancer ResearchDecember 2, 2022Eric M. Bressler, Ngoc-Quynh Chu, Robert C. Sabatelle, David A. Mahvi, Jenny T. Korunes-Miller, Fumiaki Nagashima, Fumito Ichinose, Rong Liu, Mark W. Grinstaff, Yolonda L. Colson, et al.on a biweekly basis for mycoplasma by PCR via SouthernBiotech’s mycoplasma detection kit (Cat. No. 13100-01). In Vitro Assessment of Cytotoxicity LP6 and CS1 cells were plated in 12-well plates at a seeding

(7) TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancerNature CommunicationsDecember 1, 2022Zhangli Su, Ida Monshaugen, Briana Wilson, Fengbin Wang, Arne Klungland, Rune Ougland, Anindya Duttapenicillin/streptomycin. Mycoplasma contamination was routinely checked by PCR kit (SouthernBiotech #13100-01). Cells were grown in humidified incubators with 5% CO2 at 37 °C. To construct stable HEK293T cells

(8) Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike proteinCell Host & MicrobeSeptember 14, 2022Kamyab Javanmardi et al.also tested for mycoplasma contamination before use via the Mycoplasma Detection Kit (SouthernBiotech 13100-01). Method details Automated pipeline for spike variant cloning We used a high-throughput automated

(9) Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infectionResearch squareAugust 15, 2022Hou J, Wei Y, Zou J, Jaffery R, Liang S, Zheng C, Chen K, Shi PY, Chen Y, Xie X, et al.fingerprinting or the expression of tagged markers used for genetic modification. The mycoplasma detection kit (#13100-01, SouthernBiotech, Birmingham, AL) was used to routinely monitor for mycoplasma contamination of cultured cells

(10) An Active Learning Framework Improves Tumor Variant InterpretationCancer ResearchAugust 3, 2022Alexandra M. Blee, Bian Li, Turner Pecen, Jens Meiler, Zachary D. Nagel, John A. Capra, Walter J. Chazintesting conducted on March 2021 prior to completion of the FM-HCR studies in May 2021 (SouthernBiotech #13100–01). XPA expression plasmids contain full-length human XPA (NM_000380) with the indicated mutations in

(11) Inhibition of LSD1 Attenuates Oral Cancer Development and Promotes Therapeutic Efficacy of Immune Checkpoint Blockade and YAP/TAZ InhibitionMolecular Cancer ResearchMay 4, 2022Thabet Alhousami, Michael Diny, Faiza Ali, Jennifer Shin, Gaurav Kumar, Vikas Kumar, Joshua D. Campbell, Vikki Noonan, Glenn J. Hanna, Gerald V. Denis, et al.and routine mycoplasma testing was performed using Mycoplasma detection kit from Southern Biotech (# 13100–01). Low passage number HSC-3 and CAL-27 cells were grown in 100mm3 plates for 48 to 72 hours or at confluency

(12) SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptomeeLifeMarch 16, 2022Zaffagni M, Harris JM, Patop IL, Pamudurti NR, Nguyen S, Kadener S.Scientific, 25-512). Mycoplasma test was performed using Mycoplasma Detection Kit by Southern Biotech (13100-01), according to manufacturer’s instructions. Transient plasmid transfection Plasmids pLVX-EF1alpha-SARS-CoV-2-proteins-2xStrep-IRES-Puro

(13) The pleiotropic benefits of statins include the ability to reduce CD47 and amplify the effect of pro-efferocytic therapies in atherosclerosisNat Cardiovasc ResMarch 1, 2022Jarr KU, Ye J, Kojima Y, Ye Z, Gao H, Schmid S, Luo L, Baylis RA, Lotfi M, Lopez N, et al.collected to rule out mycoplasma contamination by polymerase chain reaction (SouthernBiotech, Catalogue# 13100–01, Extended Data Figure 1d) and/or biochemical detection (Lonza, Catalogue# LT07–118 and LT07–518, Extended

(14) Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregationDevelopmental cellDecember 20, 2021Merta H, Carrasquillo Rodríguez JW, Anjur-Dietrich MI, Vitale T, Granade ME, Harris TE, Needleman DJ, Bahmanyar S.Cells were tested for mycoplasma upon initial thaw and generation of new cell lines (Southern Biotech 13100-01), and untreated cells were continuously profiled for contamination by assessment of extranuclear DAPI/Hoechst

(15) Rapid characterization of spike variants via mammalian cell surface displayMolecular CellDecember 16, 2021Kamyab Javanmardi et al.were tested for mycoplasma contamination before use via the Mycoplasma Detection Kit (SouthernBiotech 13100-01). Cells were maintained in a humidified atmosphere of 5% CO2 and 37°C and were passaged every 2-3 days

(16) Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing CentersCellsNovember 27, 2021Buchwalter RA, Ogden SC, York SB, Sun L, Zheng C, Hammack C, Cheng Y, Chen JV, Cone AS, Meckes DG Jr, et al.were tested for and free of mycoplasma using the PCR Mycoplasma Detection Kit (SouthernBiotech, cat# 13100-01). 2.2. Drug Treatments To eliminate centrosomes from SNB19 cells, cultures were treated for 7 days

(17) Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface DynamicsACS NanoNovember 23, 2021Samuel M. Leitao et al.(Gibco, Thermo Fisher Scientific). Cells were regularly tested negative for Mycoplasma (SouthernBiotech 13100-01). HeLa cells were engineered to express a doxycycline-inducible GFP fused to a mouse CD80 transmembrane

(18) Nicotinamide Mononucleotide Prevents Cisplatin-Induced Cognitive ImpairmentsCancer ResearchJuly 1, 2021Ki Hyun Yoo et al.lines were routinely tested for Mycoplasma using the Mycoplasma Detection Kit (SouthernBiotech, Cat# 13100–01) and have been confirmed to be negative. All cells were grown in DMEM/F12 media (Gibco, Cat #11330032)

(19) Respiratory and intestinal epithelial cells exhibit differential susceptibility and innate immune responses to contemporary EV-D68 isolateseLifeJuly 1, 2021Megan Culler Freeman, Alexandra I Wells, Jessica Ciomperlik-Patton, Michael M Myerburg, Liheng Yang, Jennifer Konopka-Anstadt, Carolyn B Coynewere tested for mycoplasma on a monthly basis using a sensitive PCR-based assay (Southern Biotech, 13100–01). Human intestinal enteroids Human intestinal enteroid lines were derived as previously described

(20) The Salmonella effector protein SopD targets Rab8 to positively and negatively modulate the inflammatory responseNature MicrobiologyMay 1, 2021Huan Lian, Kun Jiang, Ming Tong, Zhe Chen, Xiaoyu Liu, Jorge E. Galán, Xiang Gaoroutinely tested for mycoplasma contamination using a Mycoplasma Detection Kit (Southern Biotech, Cat# 13100-01). Plasmid construction For recombinant protein expression in E. coli the full-length sopD, sopD2 and

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